Methods and compositions for detection and analysis of...

Details Methods and compositions for detection and analysis of... Methods and compositions for detection and analysis of...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07F 5/02 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2515155

Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor PBP that can bind to the target polynucleotide. The sensor PBP comprises a signaling chromophore to absorb energy from the excited multichromophore and emit light in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.

L'invention concerne des procédés, des compositions et des articles manufacturés permettant de détecter la présence d'un polynucléotide cible dans un échantillon. Un échantillon soupçonné de contenir le polynucléotide cible est mis en contact avec un multichromophore polycationique et une protéine capteur de liaison au polynucléotide pouvant se lier au polynucléotide cible. Cette protéine capteur de liaison au polynucléotide contient un chromophore de signalisation destiné à absorber l'énergie du multichromophore excité et à émettre de la lumière en présence du polynucléotide cible. Lesdits procédés peuvent être appliqués sous forme multiplexe. Cette invention concerne également des trousses comportant des réactifs pour la mise en oeuvre desdits procédés.

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