Method of preparing a composite bone graft

A - Human Necessities – 61 – L

Patent

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Details

A61L 27/38 (2006.01) A61F 2/02 (2006.01) A61F 2/46 (2006.01) A61K 35/28 (2006.01) A61K 35/32 (2006.01) A61L 27/10 (2006.01) A61L 27/32 (2006.01) A61L 27/36 (2006.01) A61L 27/40 (2006.01) A61L 27/48 (2006.01) A61L 27/58 (2006.01) A61F 2/00 (2006.01) A61F 2/28 (2006.01) A61F 2/30 (2006.01)

Patent

CA 2291706

The present invention provides a new and improved method for preparing a composite bone graft. The method comprises providing a bone marrow aspirate suspension and passing the bone marrow aspirate suspension through a porous, biocompatible, implantable substrate (12) to provide a composite bone graft having an enriched population of connective tissue progenitor cells. Because the method is preferably performed intraoperatively, it reduces the number of occasions the graftee must undergo invasive procedures. The invention also relates to an improved composite bone graft prepared according to the present method. The improved composite graft comprises an enriched population of connective tissue progenitor cells and a greater number of connective tissue progenitor cells per unit volume than that found in the original bone marrow aspirate. The present invention also relates to a kit comprising the apparatus (10) used for preparing the composite bone graft.

La présente invention a pour objet un procédé nouveau et amélioré pour préparer un greffon osseux composite. Ce procédé consiste à fournir un produit d'aspiration de moelle osseuse en suspension et à faire passer ce produit à travers un substrat poreux, biocompatible et implantable pour constituer un greffon osseux composite comportant une population enrichie de cellules parentes de tissu conjonctif. Ce procédé peut être mis en oeuvre de manière peropératoire, ce qui réduit le nombre de procédures invasives que doit subir le greffé. L'invention concerne également un greffon osseux composite amélioré préparé selon le présent procédé. Le greffon composite amélioré comprend une population enrichie de cellules parentes de tissu conjonctif et un nombre de cellules parentes de tissu conjonctif par volume unitaire supérieur à celui observé dans le produit d'aspiration de moelle osseuse initial. La présente invention concerne également une trousse comprenant l'appareil utilisé pour préparer le greffon osseux composite.

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