Filtration and culture methods and apparatus

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 1/26 (2006.01) C12M 1/12 (2006.01)

Patent

CA 2222056

A method and apparatus for filtering microorganisms from a sample and culturing the microorganisms employs a membrane filter (30) in a filter holder (10) in which holder the membrane filter is supported on an absorbent support (26) which is maintained in an expansible compressed state against the membrane filter so that any expansion of the membrane filter when wetted by a sample does not cause bubbling of the membrane filter away from its support entrapping air bubbles and so preventing supply of nutrient from culture medium supplied to the support from reaching the microorganisms on the membrane filter.

Une méthode et un appareil pour filtrer les microorganismes dans un échantillon et cultiver les microorganismes; il s'agit d'une membrane filtrante (30) placée dans un porte-filtre (10) dans lequel la membrane filtrante repose sur un support absorbant (26) qui est maintenu dans un état comprimé expansible contre la membrane filtrante de manière que toute expansion de la membrane filtrante lorsqu'elle est mouillée par un échantillon n'entraîne pas la formation, entre la membrane filtrante et son support, de bulles d'air qui empêchent les éléments nutritifs du milieu de culture appliqué sur le support d'atteindre les microorganismes sur la membrane filtrante.

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