Methods of identifying compounds that target trna splicing...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) A61K 31/00 (2006.01) A61K 38/00 (2006.01) A61P 31/10 (2006.01) C12N 9/10 (2006.01) C12Q 1/00 (2006.01) C12Q 1/02 (2006.01) C12Q 1/18 (2006.01) C12Q 1/25 (2006.01) G01N 33/566 (2006.01) G01N 33/573 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2520510

The present invention relates to a method for screening and identifying compounds that modulate the activity of a fungal tRNA splicing endonuclease. In particular, the invention provides assays for the identification of compounds that inhibit or reduce the activity of a fungal tRNA splicing endonuclease. The methods of the present invention provide a simple, sensitive assay for high-throughput screening of libraries of compounds to identify pharmaceutical leads useful for preventing, treating, managing and/or ameliorating a fungal infection or fungal infestation or one or more symptoms thereof.

La présente invention se rapporte à un procédé de criblage et d'identification de composés modulant l'activité d'une endonucléase d'épissage d'ARNt fongique. L'invention concerne plus particulièrement des essais d'identification de composés inhibant ou réduisant l'activité d'une endonucléase d'épissage d'ARNt fongique. Les procédés de cette invention mettent en oeuvre un essai simple et sensible de criblage à haut rendement de bibliothèques de composés afin d'identifier des têtes de série pharmaceutiques que l'on utilise pour éviter, traiter, gérer et/ou améliorer une infection fongique ou une infestation fongique ou un ou plusieurs de leurs symptômes.

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