Microbiological test method and reagents

Details Microbiological test method and reagents Microbiological test method and reagents

C - Chemistry, Metallurgy

12

Q

C12Q 1/66 (2006.01) C12M 1/40 (2006.01) C12Q 1/04 (2006.01) C12Q 1/48 (2006.01)

Patent

CA 2154462

A method for determining the presence and/or amount of microorganisms and/or their intracellular material present in a sample comrpising estimating the amount of adenylate kinase therein by its ability to convert adenosine diphosphate (ADP) to adenosine triphosphate (ATP) and relating that to the presence/or amount of organism and/or intracellular material. The method provides improved sensitivity over existing luciferase/luciferin assays. Reagents including purified ADP and adenyl kinase free luciferase are provided together with test kits including these and apparatus for automated operation of the method.

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