Phosphopantetheinyl transferases and uses thereof

Details Phosphopantetheinyl transferases and uses thereof Phosphopantetheinyl transferases and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C12N 9/10 (2006.01) C12N 9/12 (2006.01) C12P 1/00 (2006.01) C12Q 1/48 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2232230

The invention pertains to isolated phosphopantetheinyl transferases, such as the E. coli acyl carrier protein synthase, which transfer a phosphopantetheinyl group onto a substrate. The enzyme can be purified from a natural source, produced recombinantly, or synthetically. Accordingly, the invention provides compositions and kits including phosphopantetheinyl transferases and host cells expressing phosphopantetheinyl transferases. The invention also provides nucleic acids encoding phosphopantetheinyl transferases and vectors comprising such nucleic acids. The invention further provides methods for phosphopantetheinylating a substrate in vitro or in vivo and methods for producing antibiotics in vitro or in vivo.

Cette invention se rapporte à des transférases de phosphopantéthéinyles, du type synthases de protéines transporteuses d'acyles dérivées de E.coli, qui transfèrent un groupe phosphopantéthéinyle à la surface d'un substrat. L'enzyme peut être obtenue par purification d'une source naturelle, par recombinaison ou par synthèse. L'invention concerne, par conséquent, des compositions et des kits contenant des transférases de phosphopantéthéinyles et des cellules hôtes exprimant des transférases de phosphopantéthéinyles. L'invention concerne en outre des acides nucléiques codant des transférases de phosphopantéthéinyles et des vecteurs comportant de tels acides nucléiques. Enfin, l'invention concerne des procédés permettant de transférer in vitro ou in vivo des groupes phosphopantéthéinyles à la surface d'un substrat ainsi que des procédés de production in vitro ou in vivo d'antibiotiques.

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