Methods and compositions for obtaining and using...

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G01N 33/53 (2006.01) C12M 1/34 (2006.01) G01N 33/543 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2648154

Methods for isolating and using multi-protein complexes that are biologically active are provided. The complexes contain one or more proteins of interest (e.g. a receptor, ion channel, etc.) and associated scaffolding proteins such as phosphatases, kinases and post synaptic density components. Buffers that do not contain denaturing agents and which may be used to isolate the multi-protein complexes are also provided, as are protein arrays containing the biologically active multi-protein complexes. The protein arrays may be used, for example, for high throughput screening assays.

L'invention porte sur des procédés qui permettent d'isoler et d'utiliser des complexes multiprotéiques à activité biologique. Les complexes de l'invention contiennent une ou plusieurs protéines d'intérêt (p.ex., un récepteur, un canal ionique, etc.) et des protéines d'échafaudage associées telles que des phosphatases, des kinases et des composants à densité post-synaptique. L'invention concerne également des tampons ne contenant pas d'agents dénaturants, qui peuvent être utilisés pour isoler les complexes multiprotéiques, et des réseaux de protéines contenant les complexes multiprotéiques précités. Les réseaux de protéines peuvent être utilisés, par exemple, dans des essais de criblage à haut rendement.

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