Purification of higher order transcription complexes from...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) A01K 67/00 (2006.01) A01K 67/027 (2006.01) C07K 1/22 (2006.01) C07K 14/435 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C07K 16/28 (2006.01) C07K 19/00 (2006.01) C12N 5/16 (2006.01) C12N 15/00 (2006.01) C12N 15/06 (2006.01) C12N 15/09 (2006.01) C12N 15/11 (2006.01) C12N 15/12 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2232806

Purification of higher order transcription complexes from transgenic non-human animals The invention relates to transgenes comprising DNA encoding for epitope-tagged TATA-box binding protein (TBP), the production of transgenic animals which express epitope-tagged TBP and use thereof for affinity purification of (novel) transcription factors and transcription complexes from a variety of eukaryotic tissues and cell-types.

Purification de complexes de transcription d'ordre supérieur à partir d'animaux transgéniques non humains. L'invention porte sur des transgènes renfermant de l'ADN codant la protéine fixatrice de boîte TATA (TBP) marquée par un épitope, sur la production d'animaux transgéniques qui expriment la TBP marquée par un épitope et sur l'utilisation de celle-ci dans la purification par affinité de facteurs de transcription et de complexes de transcription (nouveaux) à partir d'une gamme de tissus et de types cellulaires eucaryotes.

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