C - Chemistry – Metallurgy – 07 – F
Patent
C - Chemistry, Metallurgy
07
F
C07F 9/54 (2006.01) C07C 211/63 (2006.01) C09K 11/06 (2006.01) C12Q 1/68 (2006.01) G01N 33/52 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2123753
A dicationic surfactant for enhancing the chemiluminescence of chemiluminescent compounds particularly dioxetanes which are triggerable is described. The surfactant has the formula x-(R1)3A+CH2-Link-CH2A+(R2)3X- wherein A is selected from the group consisting of phosphorus and nitrogen atoms separately or together, wherein X is an anionic counterion and R1 and R2 are selected from the group consisting of unsubstituted and substituted alkyl and aralkyl groups containing 1 to 20 carbon atoms and R1 and R2 can be the same or different, and wherein Link is a carbon chain group selected from the group consisting of dialkylenearyl, aryl, alkylene, alkenylene and alkynylene groups containing 4 to 20 carbon atoms. The surfactants are particularly useful in Western and Southern blot assays.
n agent tensio-actif dicationique servant à activer l'activité de composés chimioluminescents, particulièrement des dioxétanes excitables, est décrit. Cet agent a la formule x-(R1)3A+CH2-LIEN-CH2A+(R2)3X- où A est choisi dans le groupe constitué d'atomes de phosphore et d'azote, ensemble ou séparément, où X est un anion antagoniste et où R1 et R2 sont choisis dans le groupe formé de groupements alkyle et aralkyle, substitués ou non, contenant 1 à 20 atomes de carbone; R1 et R2 peuvent être identiques ou différents; le lien est un groupe à chaîne carbonée choisi dans le groupe constitué des groupements dialkylènearyle, aryle, alkylène, alcénylène et alcynylène contenant de 4 à 20 atomes de carbone. Les agents tensio-actifs sont particulièrement utiles pour les tests de type Western ou Southern.
Akhavan-Tafti M. Hashem
Arghavani Zahra
Lumigen Inc.
Macrae & Co.
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