Method for screening an enhancer or a masker of salty taste

Details Method for screening an enhancer or a masker of salty taste Method for screening an enhancer or a masker of salty taste

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/705 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12Q 1/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2658596

The invention relates to a nucleic acid molecule being represented by a nucleic acid sequence comprising at least three nucleic acid subsequences, each nucleic acid subsequence coding for one of the, and subunits of the epithelial sodium channel, and each nucleic acid subsequence being arranged in a head to tail configuration with respect to another. The invention further relates to a cell comprising the nucleic acid sequence of the invention, which is able to express the subunits and to its use in a method for screening a potential modulator compound of the subunits of the epithelial sodium channel.

L'invention concerne une molécule d'acide nucléique représentée par une séquence d'acide nucléique comprenant au moins trois sous-séquences d'acide nucléique, chaque sous-séquence d'acide nucléique codant un des sous-unités du canal sodium épithélial, et chaque sous-séquence d'acide nucléique étant disposée selon une configuration tête-bêche. L'invention concerne également une cellule renfermant la séquence d'acide nucléique de l'invention, qui est capable d'exprimer les sous-unités ainsi que son utilisation dans un procédé de criblage d'un composé modulateur potentiel des sous-unités du canal sodium épithalial.

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