Method and device for cell lysis

Details Method and device for cell lysis Method and device for cell lysis

C - Chemistry, Metallurgy

12

N

C12N 1/06 (2006.01)

Patent

CA 2319021

The invention concerns a method and a device for cell lysis which consists in continuously producing a liquid mixture of bacteria or eukaryiotic cells and a lysis agent, and in immediately causing said mixture to flow in a constant flux inside a pipe (7), the flow rate of said flux being adapted, according to the pipe (7) length and diameter, so as to obtain a substantially homogeneous lysate at said pipe (7) outlet.

Procédé et dispositif de lyse cellulaire dans lequel on réalise, en continu, un mélange liquide de bactéries ou de cellules eucaryotes et d'un agent de lyse, et l'on fait immédiatement écouler ce mélange dans un flux constant à l'intérieur d'une canalisation (7), le débit de ce flux étant adapté, en fonction du diamètre et de la longueur de la canalisation (7), de manière à obtenir un lysat cellulaire sensiblement homogène à la sortie (8) de ladite canalisation.

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