Plant fatty acid synthases and use in improved methods for...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) C12N 9/10 (2006.01) C12N 9/16 (2006.01) C12N 15/54 (2006.01)

Patent

CA 2284286

By this invention, compositions and methods of use related to .beta.-ketoacyl- ACP synthase of special interest are synthases obtainable from Cuphea species. Amino acid and nucleic acid for synthase protein factors are provided, as well as methods to utilize such sequences in constructs for production of genetically engineered plants having altered fatty acid compositions. Of particular interest is the expression of synthase protein factors in conjunction with expression of plant medium-chain acyl-ACP thioesterases for production of increased levels and/or modified ratios of medium-chain fatty acids in oils of transgenic plant seeds.

La présente invention concerne des compositions et des procédés d'utilisation se rapportant à la synthétase .beta.-cétoacyl-ACP, avec un intérêt particulier pour les synthétases que l'on peut obtenir à partir de l'espèce Cuphea. L'invention concerne également un acide aminé et un acide nucléique pour des facteurs protéines de synthétase, ainsi que des procédés permettant d'utiliser de telles séquences dans des constructions destinées à la production de végétaux obtenus par génie génétique et où les compositions d'acides gras sont modifiées. L'invention concerne particulièrement l'expression des facteurs protéines de synthétase en relation avec l'expression d'acyl-ACP thioestérases végétales à chaîne moyenne pour la production à des niveaux plus élevés et/ou selon des rapports modifiés des acides gras à chaîne moyenne dans les huiles des graines d'origine végétale transgénique.

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