Methods of microencapsulating pancreatic islet cells

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/071 (2010.01) A61K 35/39 (2006.01) C12N 11/04 (2006.01) C12N 11/10 (2006.01) A61K 9/50 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2362022

Methods of treating and forming biocompatible microcapsules that contain living cells are provided, to improve the function of the microcapsules. In particular, methods of treating islet cells or microcapsules containing islet cells are provided. Culture of isolated islet cells prior to encapsulation, culture of encapsulated cells, and cryopreservation of islet cells prior to encapsulation, are described. Methods for harvesting viable islets that incorporates an anti-oxidant in the digestion medium are also disclosed.

La présente invention concerne des procédés de traitement et de formation de microcapsules biocompatibles contenant des cellules vivantes, destinés à améliorer la fonction des microcapsules. Elle concerne, en particulier, des procédés de traitement de cellules d'îlot ou de microcapsules contenant des cellules d'îlot. Elle concerne aussi la culture de cellules d'îlot isolées avant encapsulation, la culture de cellules encapsulées, et la cryoconservation de cellules d'îlot avant encapsulation. Elle concerne encore des procédés de récolte d'îlots viables comprenant, dans le milieu de digestion, un antioxydant.

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