C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/08 (2006.01) C11D 3/386 (2006.01) C12N 1/20 (2006.01) C12N 9/20 (2006.01)
Patent
CA 2097000
ABSTRACT A microorganism belonging to the genus Pseudomonas; an alkaline lipase produced by the microorganism or its mutants; a method of producing the alkaline lipase; and detergents containing the alkaline lipase as an aid, the alkaline lipase having (1) an operative pH of from 4 to 11.5, and an optimum pH of from 7.0 to 9.5, as measured using triolein emulsion as a substrate; (2) an operative temperature of from 10 to 80°C, and an optimum temperature of from 55 to 65°C, as measured using triolein emulsion as a substrate; (3) a molecular weight of 28,000 ? 2,000 as measured by electrophoresis using SDS polyacrylamide; (4) isoelectric point of 4.5 ? 1.5 as measured by isoelectric focusing polyacrylamide gel electrophoresis; and (5) an inhibition of lipase activity by a detergent component of not higher than 50% as measured using sodium linear- alkylbenzenesulfonate as said detergent component. The alkaline lipase has an improved stability against detergent components such as a surfactant and a protease, and can be blended with a detergent together with a protease. The alkaline lipase is less susceptible to inhibition of lipase activity by a detergent component and can increase washing power of detergents.
Goto Yukie
Mizukoshi Tatsuya
Moriya Norio
Sakai Nobuaki
Sakimoto Kazunori
Novo Nordisk A/s
Sim & Mcburney
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