Over-expression of proteins

Details Over-expression of proteins Over-expression of proteins

C - Chemistry, Metallurgy

12

N

C12N 15/67 (2006.01) C12N 1/20 (2006.01) C12N 15/64 (2006.01) C12N 15/70 (2006.01) C12P 21/02 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2255683

The invention describes a method for selecting host cell mutants which are resistant to expression system toxicity, comprising the steps of growing an expression system comprising host cells transformed with an expression vector, inducing the expression system such that a toxic effect is observed, and selecting viable cells in which the expression vector continues to function. Cells thus obtained are useful for the expression of polypeptide gene products in microorganisms.

L'invention décrit une méthode pour choisir des mutants de cellules hôtes, résistant à la toxicité d'un système d'expression. La méthode comprend les étapes suivantes : croissance d'un système d'expression renfermant des cellules hôtes transformées avec un vecteur d'expression; induction du système d'expression de façon à observer un effet toxique; sélection de cellules viables dans lesquelles le vecteur d'expression continue à fonctionner. Les cellules ainsi obtenues sont utiles pour l'expression de produits géniques polypeptidiques chez les microorganismes.

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