Method for detecting and counting micro-organisms in a sample

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/04 (2006.01) C12Q 1/06 (2006.01)

Patent

CA 2500464

The invention concerns a method for detecting and counting micro-organisms in a sample comprising the following steps: (a) selectively enriching the micro-organism sought in the sample, (b) conditioning said micro-organism, (c) immunomagnetic concentration of the conditioned micro-organism, (d) fluorescent marking of the concentrated micro-organism, and (e) detecting and analyzing the fluorescence.

Procédé de détection et de numération de microorganismes dans un échantillon comprenant les étapes de : (a) enrichissement sélectif du microorganisme recherché dans l'échantillon,(b) conditionnement dudit microorganisme, (c) concentration immunomagnétique du microorganisme conditionné, (d) marquage fluorescent du microorganisme concentré, et (e) détection et analyse de la fluorescence.

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