Detecting and amplifying target nucleic acids using...

Details Detecting and amplifying target nucleic acids using... Detecting and amplifying target nucleic acids using...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2140331

The present invention relates to improved LCR amplification schemes using at least one downstream probe modified at its 5' end to reduce or eliminate target independent amplification. The different modified probes, and kits containing them are also presented. Also presented is a method for detecting differences in nucleic acid sequences, with reduced target independent amplification, using the modified probes.

La présente invention se rapporte à des procédés d'amplification en chaîne par ligase (LCR), consistant à utiliser au moins une sonde en aval modifiée au niveau de son extrémité 5' afin de réduire ou de supprimer l'amplification indépendante de la cible. Les différentes sondes modifiées, des kits les contenant, sont également décrites, ainsi que des procédés permettant de détecter des différences dans des séquences d'acide nucléique, ces procédés présentant une amplification indépendante de la cible réduite, et consistant à utiliser les sondes modifiées.

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