Method for producing insulin from a natural source and insulin

Details Method for producing insulin from a natural source and insulin Method for producing insulin from a natural source and insulin

C - Chemistry, Metallurgy

07

K

C07K 14/625 (2006.01)

Patent

CA 2539380

The inventive method for producing insulin consists in using a reindeer pancreas which is homogenised in a hydrochloride ethanol solution. Afterwards, the extraction associated with the subsequent clarification of the solution and a supernatant production is carried out. Said supernatant is exposed to an ion-exchange chromatography and isoelectric precipitation, thereby producing insulin which is purified by means of a high-efficient reversed-phase liquid chromatography. The thus produced insulin can compete for a bond with an insulin receptor at a concentration higher than 100 ng/ml.

Pour fabriquer de l'insuline on utilise le pancréas de rennes, homogénéisé dans une solution d'éthanol chlorhydrique, on effectue une extraction et un éclaircissement de la solution et l'on obtient un surnageant qui est soumis à la chromatographie par échange d'ions et à la sédimentation isoélectrique; ces opérations permettant d'obtenir une insuline qui est purifiée par chromatographie liquide en phase inversée à efficacité élevée. L'insuline ainsi obtenue peut entrer en concurrence pour la liaison avec le récepteur d'insuline à une concentration supérieure à 100 ng/ml.

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