Cytochrome p450 monooxygenase and nadph cytochrome p450...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/53 (2006.01) C07K 14/40 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/81 (2006.01) C12P 7/44 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2349868

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzyme of the w-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

La présente invention concerne des gènes isolés qui codent pour des enzymes du cytochrome P450 et de réductase de NADPH du complexe .omega.-hydroxylase de C. tropicalis 20336. L'invention concerne aussi des vecteurs contenant ces gènes, des cellules hôtes transfectées ainsi que des cellules hôtes transformées. Sont aussi décrits des procédés de production des enzymes du cytochrome P450 et de réductase de NADPH qui consistent à transformer une cellule hôte à l'aide d'un gène qui code pour ces enzymes et à cultiver les cellules. L'invention concerne aussi des procédés d'augmentation de la production d'un acide dicarboxylique, des procédés d'augmentation de la production des enzymes mentionnées ci-dessus qui consistent à augmenter le nombre de gènes qui codent pour ces enzymes dans les cellules hôtes, ainsi qu'une méthode destinée à distinguer des éléments d'une famille de gènes en quantifiant l'expression des gènes.

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