C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/58 (2006.01) C12N 15/52 (2006.01) C12N 15/66 (2006.01) C12N 15/67 (2006.01) C12N 15/80 (2006.01) C12P 21/00 (2006.01)
Patent
CA 2435606
The invention concerns a method for the expression of a gene coding for a soluble proteinase K in yeast e.g. in Pichia pastoris with subsequent secretion into the culture medium. In addition a method for purifying the heterologously expressed and secreted proteinase K is described.
L'invention concerne un procédé permettant d'exprimer de manière soluble un gène codant la protéinase K dans de la levure, par ex. dans la pichia pastoris, puis de le sécréter dans le milieu de culture. L'invention concerne en outre un procédé de purification de la protéinase K exprimée et sécrétée de manière hétérologue.
Geipel Frank
Glaser Stephan
Hoelke Werner
Kirschbaum Thomas
Mueller Rainer
Borden Ladner Gervais Llp
F. Hoffmann-La Roche Ag
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