Microorganism detection method and apparatus

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/01 (2006.01) C12N 15/65 (2006.01) C12N 15/74 (2006.01) C12Q 1/04 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2684311

Embodiments of the present invention relate to selective organism detection, and, more particularly to recombinant bacteriophages and the use of such recombinant bacteriophages to detect target bacteria and to detect specific nucleic acid sequences within said target bacteria thus allowing for the detection of phenotypic characteristics of said bacteria such as determining drug(s) to which such target bacteria are resistant. The present invention further relates to sample preparation apparatuses for preparing samples for detection and analysis using bacteriophage-based techniques, that are low in cost, easy to use, and do not require technical expertise or any additional laboratory infrastructure to perform.

La présente invention concerne, dans des modes de réalisation, une détection d'organisme sélective et, plus particulièrement, des bactériophages recombinants, et l'utilisation de tels bactériophages recombinants pour détecter des bactéries cibles et pour détecter des séquences d'acide nucléique spécifique dans ladite bactérie cible en permettant ainsi la détection de caractéristiques phénotypiques de ladite bactérie, telles la détermination de médicaments auxquels de telles bactéries cibles sont résistantes. La présente invention concerne en outre des appareils de préparation d'échantillon afin de préparer des échantillons pour une détection et une analyse à l'aide de techniques fondées sur des bactériophages, qui sont peu coûteuses, faciles à utiliser et ne nécessitent pas d'expertise technique ou tout autre infrastructure de laboratoire supplémentaire.

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