Method for expressing polypeptides in cyanobacteria

C - Chemistry – Metallurgy – 12 – N

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C12N 15/74 (2006.01) C12N 1/13 (2006.01) C12N 9/02 (2006.01) C12N 15/53 (2006.01)

Patent

CA 2082802

A method for expressing a physiologically active polypeptide in cells of a blue-green alga by transforming the cells of a blue-green alga with a recombinant DNA containing a structural gene encoding the polypeptide, characterized in that as the recombinant DNA is used a recombinant DNA which contains a structural gene encoding the physiologically active polypeptide, the transcription initiation region of the RuBisCO gene of Anacystis nidulans located upstream of the structural gene, and the transcription termination region of the RuBisCO gene located downstream of the structural gene. According to this method, physiologically active polypeptide can efficiently be expressed using blue-green algae.

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