Systems and methods for altering rates of enzymatic processes

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 19/14 (2006.01) C12M 1/02 (2006.01) C12M 1/42 (2006.01) C12N 9/24 (2006.01) C12P 1/00 (2006.01) C12P 19/00 (2006.01)

Patent

CA 2709425

The various embodiments of the present invention relate generally to compositions, systems, and methods for altering rates of catalysis. More particularly, the various embodiments of the present invention are directed toward compositions, systems, and methods for enzymatic hydrolysis of polysaccharides, such as cellulose and starch. An aspect of the present invention comprises a method for altering the rate of conversion of a substrate into a product comprising: providing a substrate in a carrier; mixing a reactant and a co-factor with the carrier to form a substantially homogeneous mixture of the reactant, the co-factor, and the substrate in the carrier; and reacting the reactant with the substrate in the presence of the co- factor to convert at least a portion of the substrate into the product, wherein the reaction rate of the reactant with the substrate in the presence of the co-factor is different than the reaction rate of the reactant with the substrate in the absence of the co-factor.

Les modes de réalisation de la présente invention concernent en général des compositions, des systèmes, et des procédés pour modifier les vitesses de catalyse. Plus particulièrement, les divers modes de réalisation de la présente invention concernent des compositions, des systèmes, et des procédés d'hydrolyse enzymatique de polysaccharides, tels que la cellulose et l'amidon. Un aspect de la présente invention comprend un procédé pour modifier la vitesse de conversion d'un substrat en un produit comprenant les étapes consistant à : fournir un substrat dans un transporteur ; mélanger un réactif et un cofacteur avec le transporteur afin de former un mélange essentiellement homogène du réactif, du cofacteur, et du substrat dans le transporteur ; et faire réagir le réactif avec le substrat en présence du cofacteur afin de convertir au moins une partie du substrat en le produit, la vitesse de réaction du réactif avec le substrat en présence du cofacteur étant différente de la vitesse de réaction du réactif avec le substrat en l'absence du cofacteur.

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