Polynucleotide sequencing using a helicase

Details Polynucleotide sequencing using a helicase Polynucleotide sequencing using a helicase

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2367277

A method for sequencing a polynucleotide, comprises the steps of: (i) reacting a target polynucleotide with a helicase/primase enzyme (which may be immobilised), under conditions suitable for enzyme activity; and (ii) detecting the interaction between the enzyme and a nucleotide on the target, by measuring radiation.

L'invention concerne un procédé de séquençage de polynucléotide comprenant les étapes suivantes: (i) réaction entre un polynucléotide cible et une enzyme hélicase/primase (susceptible d'être immobilisée), dans des conditions appropriées à l'activité enzymatique; et détection de l'interaction entre l'enzyme et un nucléotide sur la cible, par mesure du rayonnement.

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