Exo-endo cellulase fusion protein

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/56 (2006.01) C12N 9/42 (2006.01) C12N 15/62 (2006.01) C12N 15/80 (2006.01) C12P 21/02 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2560593

The present invention relates to a heterologous exo-endo cellulase fusion construct, which encodes a fusion protein having cellulolytic activity comprising a catalytic domain derived from a fungal exo-cellobiohydrolase and a catalytic domain derived from an endoglucanase. The invention also relates to vectors and fungal host cells comprising the heterologous exo-endo cellulase fusion construct as well as methods for producing a cellulase fusion protein and enzymatic cellulase compositions.

Un gène hybride de fusion de cellulase exo-endo hétérologue codant une protéine de fusion ayant une activité cellulotique comprenant un domaine catalytique dérivé d'une exo- cellobiohydrolase fongique et un domaine catalytique dérivé d'une endoglucanase. Des vecteurs et des cellules hôtes fongiques comprennent le gène hybride de fusion de cellulase exo-endo hétérologue ainsi que des procédés de production d'une protéine de fusion de cellulase et des compositions de cellulase enzymatiques.

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