Enzymatically-active recombinant human .beta.-tryptase and...

Details Enzymatically-active recombinant human .beta.-tryptase and... Enzymatically-active recombinant human .beta.-tryptase and...

C - Chemistry, Metallurgy

12

N

C12N 15/81 (2006.01) C07K 16/40 (2006.01) C12N 1/19 (2006.01) C12N 9/64 (2006.01) C12N 15/57 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2326700

Disclosed is a method of expressing enzymatically-active, recombinant human .beta.-tryptase in a yeast host cell, expression constructs which drive the production of enzymatically-active tryptase in transformed yeast, and genetically-engineered yeast containing the expression constructs and which express enzymatically-active human .beta.-tryptase. Uses for the tryptase so produced are also disclosed.

L'invention a trait à une méthode d'expression de la .beta.-tryptase humaine de recombinaison active du point de vue enzymatique dans une cellule de levure hôte, à des produits de recombinaison d'expression commandant la production de cette tryptase dans la levure transformée ainsi qu'à de la levure génétiquement modifiée contenant ces produits de recombinaison d'expression et exprimant la .beta.-tryptase humaine de recombinaison active du point de vue enzymatique. Cette invention concerne également des applications de la tryptase ainsi produite.

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