Detecting receptor oligomerization

Details Detecting receptor oligomerization Detecting receptor oligomerization

G - Physics

01

N

G01N 33/566 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2490654

Methods are provided for detecting formation of oligomeric complexes of molecules on the surface of cell membranes. These methods employ pairs of tagged probes and cleaving probes, each of which binds specificly to a cell surface molecule. The tagged probe includes a molecular tag that is linked to a first binding compound through a cleavable linkage, and the cleaving probe includes a second binding agent and a cleavage-inducing moiety that can cleave the linkage when within a defined proximity thereto. Binding of the two probes to cell surface molecules that have formed an oligomeric complex results in release of the molecular tag from the binding compound, providing a measure of formation of the complex.

L'invention concerne des procédés de détection de formation de complexes oligomères de molécules sur la surface de membranes de cellules. Ces procédés utilisent des paires de sondes marquées et de sondes de scission, chacune d'elle se liant spécifiquement à une molécule de surface cellulaire. La sonde marquée comporte un marqueur moléculaire lié à un premier composé de liaison via une liaison clivable, et la sonde de scission comporte un second agent de liaison et un groupe induisant le clivage, pouvant scinder la liaison à l'intérieur d'une zone de proximité définie. La liaison des deux sondes aux molécules de surface cellulaire qui ont formé un complexe oligomère résulte dans la libération du marqueur moléculaire du composé de liaison, ce qui permet d'obtenir une mesure de la formation du complexe.

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