Neisseria gonorrhoeae detection

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2560390

A method for determining whether a human individual is or has been infected with Neisseria gonorrhoeae, is provided. The method detects a Neisseria gonorrhoeae, porA nucleic acid fragment obtained from a biological sample. The method includes subjecting the biological sample to nucleic acid sequence amplification using primers having respective nucleotide sequences according to SEQ ID NO:1 and SEQ ID NO:2, to thereby produce a porA Neisseria gonorrhoeae, amplification product. The amplification product is detected by fluorescence resonance energy transfer using oligonucleotides having respective nucleotide sequences according to SEQ ID NO:3 which has a donor fluorophore and SEQ ID NO:4, which has an acceptor fluorophore.

L'invention concerne une méthode permettant de déterminer si un individu humain est ou a été infecté par le Neisseria gonorrhoeae. La méthode permet de détecter un Neisseria gonorrhoeae, un fragment d'acide nucléique porA obtenu à partir d'un échantillon biologique. La méthode consiste à soumettre l'échantillon biologique à une amplification de séquence d'acide nucléique à l'aide d'amorces présentant des séquences nucléotidiques respectives selon la SEQ ID NO:1 et la SEQ ID NO:2, afin de produire ainsi un produit d'amplification d'acide nucléique de porA à partir de Neisseria gonorrhoeae. Le produit d'amplification est détecté par transfert d'énergie par résonance de fluorescence à l'aide d'oligonucléotides présentant des séquences nucléotidiques respectives selon la SEQ ID NO:3 qui présente un fluorophore donneur et la SEQ ID NO:4, qui présente un fluorophore accepteur.

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