Mutant dna polymerases with improved pyrophosphorolysis...

Details Mutant dna polymerases with improved pyrophosphorolysis... Mutant dna polymerases with improved pyrophosphorolysis...

C - Chemistry, Metallurgy

12

N

C12N 9/12 (2006.01)

Patent

CA 2706999

Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

L'invention concerne des ADN polymérases mutantes dont les vitesses d'extension sont améliorées par rapport à celle d'une polymérase correspondante non modifiée. Les polymérases mutants sont utiles dans divers procédés d'extension d'amorce décrits. L'invention concerne également des compositions associées, notamment des acides nucléiques, des vecteurs et des cellules hôtes recombinants, qui sont utiles, par exemple, pour la production des ADN polymérases mutantes.

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