Method for measuring cellular adhesion

Details Method for measuring cellular adhesion Method for measuring cellular adhesion

G - Physics

01

N

G01N 33/53 (2006.01) G01N 33/52 (2006.01) G01N 33/543 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2347553

A diagnostic method for determining the binding capacity of cells such as platelets or progenitors thereof in a biological sample comprises passing the biological sample over an adhesive substrate immobilised on a solid support under defined flow conditions and for a time sufficient to enable cells from the biological sample to bind to the adhesive substrate, and subsequently detecting cells bound to the adhesive substrate. The method may be used for detection in an individual of the presence of or risk of developing a condition or disorder involving cellular, particularly platelet, abnormalities.

L'invention porte sur un procédé diagnostique permettant de déterminer la capacité d'adhérence de molécules telles que des plaquettes ou leurs progéniteurs à un échantillon biologique consistant à faire passer l'échantillon sur un substrat adhésif immobilisé sur un support solide dans des conditions définies d'écoulement et pendant un temps suffisant pour permettre aux cellules de se fixer au substrat adhésif, puis à détecter les cellules s'y étant fixées. Le procédé peut servir à détecter chez un individu la présence ou le risque de développement d'un état ou d'un trouble lié à des anomalies de cellules et en particulier de plaquettes.

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