Methods for assembling multiple expression constructs

Details Methods for assembling multiple expression constructs Methods for assembling multiple expression constructs

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2564039

The present invention relates to methods for assembling constructs with multiple gene expression cassettes by directed recombinational cloning. DNA and vectors having engineered recombination sites are provided for use in a recombinational cloning method that enables efficient and specific assembly of expression cassettes to one multiple expression construct in a single recombinational cloning step.

L'invention concerne des procédés qui permettent d'assembler des produits de synthèse avec de multiples cassettes d'expression génique par clonage recombinatoire dirigé. L'invention se rapporte à des ADN et des vecteurs possédant des sites de recombinaison modifiés qui sont utilisés selon le procédé de clonage recombinatoire afin de permettre un assemblage spécifique et efficace des cassettes d'expression en un produit d'expression multiple, en une seule étape de clonage recombinatoire.

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