Purification of recombinantly produced interferon

Details Purification of recombinantly produced interferon Purification of recombinantly produced interferon

C - Chemistry, Metallurgy

07

K

C07K 14/56 (2006.01)

Patent

CA 2746502

The present invention provides a method for separating desired interferon isoforms from undesired interferon isoforms that involves subjecting the isoforms to anion exchange column chromatography and a biphasic elution procedure. A strong elution solution is used in the first elution phase to facilitate elution of the desired isoform from the column and a weak elution solution is used in the second phase to suppress elution of the desired isoforms.

La présente invention concerne un procédé permettant de séparer des isoformes d'interféron souhaitées d'isoformes d'interféron non souhaitées qui implique de soumettre les isoformes à une chromatographie sur colonne échangeuse d'anions et une procédure d'élution biphasique. Une solution d'élution forte est utilisée dans la première phase d'élution pour faciliter l'élution de l'isoforme souhaitée de la colonne et une solution d'élution faible est utilisée dans la seconde phase pour supprimer l'élution des isoformes souhaitées.

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