Method of identifying n-terminal probnp

Details Method of identifying n-terminal probnp Method of identifying n-terminal probnp

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) C07K 14/58 (2006.01) C07K 16/18 (2006.01) C07K 16/26 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2359667

The invention relates to a method of identifying N-terminal proBNP in a sample with at least two antibodies that detect different epitopes of the N-terminal proBNP. The method is used to differentiate or classify samples of healthy individuals and samples of patients of NYHA classes I to IV. The invention further relates to recombinant N-terminal proBNP, its use as standard in a method of identifying N-terminal proBNP, to antibodies that detect recombinant N-terminal proBNP and to their production.

L'invention concerne un procédé d'identification de proBNP N-terminal (propeptide natriurétique du cerveau N-terminal) dans un échantillon ayant au moins deux anticorps détectant les différents épitopes de proBNP N-terminal. Le procédé peut être utilisé pour la différenciation ou la classification d'échantillons d'individus sains et d'échantillons de patients des classes NYHA I à IV. En outre, l'invention concerne un proBNP N-terminal recombinant, son utilisation comme étalon dans un procédé d'identification de proBNP N-terminal, ainsi que des anticorps détectant le proBNP N-terminal recombinant et leur fabrication.

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