Novel enterokinase cleavage sequences

Details Novel enterokinase cleavage sequences Novel enterokinase cleavage sequences

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C07K 7/06 (2006.01) C07K 14/47 (2006.01) C07K 19/00 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2408630

Novel enterokinase cleavage sequences are provided. Also disclosed are methods for the rapid isolation of a protein of interest present in a fusion protein construct including a novel enterokinase cleavage sequence of the present invention and a ligand recognition sequence for capturing the fusion construct on a solid substrate. Preferred embodiments of the present invention show rates of cleavage up to thirty times that of the known enterokinase cleavage substrate (Asp)4-Lys-Ile.

L'invention concerne de nouvelles séquences de clivage d'entérokinase. L'invention concerne également des procédé permettant l'isolement rapide d'une protéine d'intérêt présente dans une protéine de fusion hybride contenant une nouvelle séquence de clivage d'entérokinase de la présente invention et une séquence de reconnaissance de ligand pour la capture de la protéine de fusion hybride sur un substrat solide. Des modes de réalisation préférés montrent des vitesses de clivage jusqu'à trente fois supérieures à celles du substrat de clivage d'entérokinase connu (Asp)¿4?-Lys-Ile.

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