C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/11 (2006.01)
Patent
CA 2685252
This invention provides methods, compositions and kits for rapid determination of the delivery of exogenous agents both in vitro and in vivo, including without limitation siRNA, microRNA, a ribozyme or an antisence molecule, any of which may target, bind to, or inactivate the mRNA of the gene of interest expressed in the cells. The methods, compositions and kits utilize a promoter-reporter construct whereby successful non-viral nucleic acid delivery leads to an up-regulation of reporter signals thus providing a quantitative, sensitive and rapid means of detection, validation and monitoring.
L'invention concerne des procédés, des compositions et des trousses pour la détermination rapide de l'administration d'agents exogènes in vitro et in vivo, incluant, sans limitation, de l'ARNsi, du micro-ARN, un ribozyme ou une molécule antisens, n'importe lequel pouvant cibler, se lier à, ou inactiver l'ARNm du gène d'intérêt exprimé dans les cellules. Les procédés, les compositions et les trousses utilisent une construction de promoteur-rapporteur par laquelle l'administration d'acide nucléique non viral réussie mène à une régulation à la hausse de signaux de rapporteurs pour ainsi fournir des moyens quantitatifs, sensibles et rapides de détection, de validation et de surveillance.
Fesik Steve
Li Leiming
Lin Xiaoyu
Shen Yu
Abbott Laboratories
Torys Llp
LandOfFree
Methods for assessing the delivery of small inhibitory rna... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for assessing the delivery of small inhibitory rna..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for assessing the delivery of small inhibitory rna... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1442347