Methods of directly amplifying nucleic acids from crude samples

Details Methods of directly amplifying nucleic acids from crude samples Methods of directly amplifying nucleic acids from crude samples

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2688571

The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification.

L'invention concerne des procédés, nécessaires et mélanges de réaction améliorés pour amplifier des acides nucléiques. Dans certains modes de réalisation, une formulation tampon directe novatrice est proposée, laquelle permet l'amplification directe des acides nucléiques dans un échantillon brut avec une purification minimale d'échantillon.

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