C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) C07K 14/00 (2006.01) C07K 14/51 (2006.01) C07K 14/52 (2006.01) C07K 14/53 (2006.01) C07K 14/54 (2006.01) C07K 19/00 (2006.01) C12N 1/21 (2006.01) C12N 15/67 (2006.01) C12N 15/70 (2006.01)
Patent
CA 2093643
This invention provides a fusion molecule comprising a DNA sequence encoding a thioredoxin-like protein fused to the DNA sequence encoding a selected heterologous peptide or protein. The peptide or protein may be fused to the amino terminus of the thioredoxin-like molecule, the carboxyl terminus of the thioredoxin- like molecule, or within the thioredoxin-like molecule, for example at the active-site loop of said molecule. Expression of this fusion molecule under the control of a regulatory sequence capable of directing its expression in a desired host cell, produces high levels of stable and soluble fusion protein. The fusion pro- tein, located in the bacterial cytoplasm, may be selectively released from the cell by osmotic shock or freeze/thaw procedures. It may be optionally cleaved to liberate the soluble, correctly folded heterologous protein from the thioredoxin-like portion.
Lavallie Edward R.
Mccoy John
Genetics Institute Inc.
Genetics Institute Llc
Gowling Lafleur Henderson Llp
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