Process for the determination by means of free radicals, of...

C - Chemistry – Metallurgy – 12 – Q

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150/13, 150/16

C12Q 1/02 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2025316

- 22 - ABSTRACT OF THE DISCLOSURE The present invention relates to a novel method of assaying or evaluating the antioxidizing activities of a living organism or a potentially aggressive agent, said method, which comprises using free radicals as a means of inducing cell lysis. being characterized in that 1°) a free radical generator is brought into contact. in an appropriate liquid biological medium, with a cell material selected from the group consisting of (a) human, animal and plant cells, (b) fragments of said cells, and (c) synthetic walls and fragments thereof con- taining liposomes, said cell material having first been contaminated with a potentially aggressive agent; 2°) the release of free radicals from said free radical generator is induced; and 3°) the lysis of the cell material by the free radicals is evaluated by comparison with a control con- taining said cell material which has not been contami- nated.

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