Polynucleotide encoding 2-hydroxyisoflavanone dehydratase...

Details Polynucleotide encoding 2-hydroxyisoflavanone dehydratase... Polynucleotide encoding 2-hydroxyisoflavanone dehydratase...

C - Chemistry, Metallurgy

12

N

C12N 15/60 (2006.01) A01H 5/00 (2006.01) C07H 21/00 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 9/88 (2006.01) C12N 15/00 (2006.01) C12N 15/63 (2006.01) C12P 17/02 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2520532

2-Hydroxyisoflavanone dehydratase substantially having the amino acid sequence represented by SEQ ID NO:1 is isolated from licorice. Further, a polynucleotide encoding 2-hydroxyisoflavanone dehydratase of the SEQ ID NO:2 is obtained. Furthermore, the amino acid sequence of 2-hydroxyisoflavanone dehydratase is identified from soybean and a polynucleotide encoding 2- hydroxyisoflavanone dehydratase is obtained.

Selon la présente invention, on isole à partir d'un extrait de réglisse une 2-hydroxyisoflavanone déhydratase présentant sensiblement la séquence d'acides aminés représentée par la SEQ ID NO :1. On obtient en outre un polynucléotide codant une 2-hydroxyisoflavanone déhydratase de la SEQ ID NO :2. L'invention permet en outre d'identifier la séquence d'acides aminés de la 2-hydroxyisoflavanone déhydratase à partir du soja et d'obtenir un polynucléotide codant une 2-hydroxyisoflavanone déhydratase.

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