Multi plasmid system for the production of influenza virus

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/63 (2006.01)

Patent

CA 2551489

Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. In addition, the present invention includes an improved method of rescue, wherein animal cells (e.g., SF Vero cells) are electroporated with plasmids and vectors of the invention.

La présente invention a trait à des vecteurs et des procédés pour la production de virus de la grippe appropriés en tant que vaccins recombinants de la grippe en culture cellulaire. L'invention a trait à des vecteurs d'expression bidirectionnelle destinés à être utilisés dans un système à plasmides multiples d'expression du virus de la grippe. L'invention a trait en outre à des procédés de production de virus de la grippe avec une capacité accrue de réplication dans des oeufs embryonnés et/ou de cellules de poulets (par exemple, des cellules Vero et/ou MDCK) et également à des virus de la grippe avec des caractéristiques de réplication améliorées. En outre, la présente invention a trait à un procédé amélioré de sauvetage, dans lequel des cellules animales (par exemple, des cellules souches SF Vero) sont soumises à une électroporation avec des plasmides et des vecteurs de l'invention.

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