G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/53 (2006.01) C07K 19/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/533 (2006.01)
Patent
CA 2523466
The present invention provides probes for detection and quantification of a lipid second messenger, which comprises: a polypeptide specifically bound to the lipid second messenger; two chromophores respectively having different fluorescence wavelengths, wherein each of the chromophores is linked to each end of the polypeptide through a rigid linker sequence; and a membrane localization sequence linked to one of the chromophores through a rigid linker sequence. According to the present invention, it is now possible to quantitatively detect when and in which site of a living cell the lipid second messengers are produced.
L'invention concerne une sonde pour la détection et la quantification d'un second messager lipidique, caractérisée en ce que ladite sonde comporte un polypeptide présentant une liaison spécifique avec le second messager lipidique, et deux chromophores, reliés aux deux extrémités du polypeptide au moyen des séquences de liaison rigides, qui présentent des longueurs d'ondes fluorescentes différentes l'une de l'autre, ainsi qu'une séquence de localisation de membrane, reliée à l'un des deux chromophores au moyen d'une séquence de liaison rigide. Selon l'invention, l'utilisation de ladite sonde permet une détection et une quantification d'un second messager lipidique, c.-à-d. l'identification du site de production et du temps de production de ce messager dans les cellules vivantes.
Sato Moritoshi
Umezawa Yoshio
Japan Science And Technology Agency
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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