C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/02 (2006.01) C12Q 1/04 (2006.01)
Patent
CA 2748566
The present invention provides a method of evaluating the possibility of a stem cell being able to differentiate into a cell that constitutes a desired tissue in a living organism, comprising the following steps of: (1) transplanting a stem cell to be evaluated into a primordium of a desired tissue of a non-human mammal, (2) culturing the tissue primordium in vitro, and (3) determining the possibility that the stem cell differentiates into a cell that constitutes the tissue in a living organism, with the degree of the dispersion of cells derived from the transplanted stem cell in the cultured tissue primordium as an index.
L'invention porte sur un procédé pour évaluer la possibilité de différenciation d'une cellule souche en une cellule constituant un tissu désiré in vivo. Le procédé comprend les étapes suivantes (1) à (3) consistant à : (1) transplanter une cellule souche devant être évaluée dans un tissu primordial désiré issu d'un mammifère non humain ; (2) mettre en culture le tissu primordial in vitro ; et (3) déterminer la possibilité de différenciation de la cellule souche en une cellule constituant le tissu in vivo par l'utilisation, en tant que mesure, du degré de dispersion des cellules issues de la cellule souche transplantée dans le tissu primordial cultivé.
No affiliations
Fetherstonhaugh & Co.
Jichi Medical University
Otsuka Pharmaceutical Factory Inc.
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