Process for purifying human papillomavirus virus-like particles

Details Process for purifying human papillomavirus virus-like particles Process for purifying human papillomavirus virus-like particles

C - Chemistry, Metallurgy

12

N

C12N 7/02 (2006.01) A61K 39/12 (2006.01) C12N 7/04 (2006.01)

Patent

CA 2339034

A process for purifying papillomavirus virus-like particles (VLPs) includes the step of passing a partially purified VLP-containing solution through a hydroxyapatite chromatography column. The VLPs are then eluted using a buffer containing phosphate anion. The advantages of this method include the recovery of a high yield of intact VLPs.

L'invention concerne un procédé permettant de purifier des particules de type papillomavirus (VLP), qui consiste à faire passer une solution contenant des VLP partiellement purifiés à travers une colonne de chromatographie d'adsorption sur gel d'hydroxyapatite. Les VLP sont ensuite élués à l'aide d'un tampon contenant des anions phosphates. Les avantages de ce procédé comprennent la récupération d'un grand nombre de VLP intacts.

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