Cell line comprising the promoter of cyclooxygenase-2...

Details Cell line comprising the promoter of cyclooxygenase-2... Cell line comprising the promoter of cyclooxygenase-2...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 9/02 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2378921

The cell line comprises a DNA construction which includes all or part of a promoter sequence of the cyclooxygenase-2 (cox-2) gene and a witness gene, operatively jointed to each other, so that said promoter sequence of the cox-2 gene directs the expression of said witness gene in response to an adequate stimulus. The assay method comprises contacting said cell line with the compound to be assayed and determining the existence of a signal which is indicative of the expression of the activity due to the witness gene. Said method is appropriate for searching cox-2 transcriptional induction selective inhibitors through appropriate stimuli.

L'invention concerne une lignée cellulaire comprenant une construction d'ADN comprenant la totalité ou une partie d'une séquence promotrice du gène de la cyclooxygénase 2 (cox 2) et un gène témoin, liés opérationnellement de manière que cette séquence promotrice du gène de la cox 2 dirige l'expression de ce gène témoin en réponse à un stimulus adéquat. Ce procédé d'analyse consiste à mettre cette lignée cellulaire en contact avec le composé à analyser et à déterminer l'existence d'un signal, indiquant l'expression de l'activité, émis par le gène témoin. Ce procédé permet de rechercher des inhibiteurs sélectifs de l'induction au niveau de la transcription de la cox 2 par stimulus appropriés.

Affiliated with

Also associated with

Rating

Cell line comprising the promoter of cyclooxygenase-2... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cell line comprising the promoter of cyclooxygenase-2..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell line comprising the promoter of cyclooxygenase-2... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1453821