Dna polymerase-related factors

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/54 (2006.01) C07K 14/195 (2006.01) C12N 9/12 (2006.01) C12N 9/22 (2006.01) C12N 15/31 (2006.01) C12N 15/55 (2006.01) C12P 19/34 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2295306

A thermostable DNA polymerase-related factor promoting the DNA synthesizing activities of DNA polymerases; a thermostable DNA polymerase-related factor having the activity of binding to DNA polymerases and a process for producing the same; genes encoding the above DNA polymerase-related factors; a method for synthesizing DNAs wherein DNA polymerases are used in the presence of the above DNA polymerase-related factors; and kits containing the above DNA polymerase- related factors. Use of these DNA polymerase-related factors makes it possible to provide DNA synthesis systems and DNA amplification systems in vitro which are superior to the conventional ones.

L'invention concerne un facteur thermostable apparenté à l'ADN polymérase, favorisant la synthétisation de l'ADN des ADN polymérases. Cette invention concerne également un facteur thermostable apparenté à l'ADN polymérase, capable de se fixer aux ADN polymérases, ainsi que son procédé de production. Cette invention concerne en outre les gènes codant les facteurs apparentés à l'ADN polymérase, un procédé de synthétisation des ADN selon lequel les ADN polymérases sont utilisées en présence desdits facteurs apparentés à l'ADN polymérase, et des kits contenant ces facteurs apparentés à l'ADN polymérase. L'utilisation de ces facteurs apparentés à l'ADN polymérase permet d'obtenir des systèmes de synthèse et d'amplification de l'ADN in vitro supérieurs aux systèmes traditionnels.

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