A method of preparing a separation matrix

B - Operations – Transporting – 01 – J

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B01J 20/30 (2006.01) B01D 15/08 (2006.01) B01J 20/285 (2006.01)

Patent

CA 2559777

The present invention is a method of preparing a separation matrix, which method comprises providing unsaturated monomers comprising one or more chromatography functionalities; contacting said monomer(s) with initiator and catalyst; performing controlled radical polymerisation of said monomers; and coupling of the resulting polymers to a base matrix. The controlled polymerisation technique may e.g. be ATRP, RAFT or NMP. The method allows preparation of well characterised ligands useful e.g. for HIC or any other kind of chromatography.

L'invention concerne un procédé d'élaboration de matrice de séparation : fourniture de monomères insaturés à une ou plusieurs fonctionnalités de chromatographie; contact entre le ou les monomères et un initiateur et un catalyseur; polymérisation radicalaire contrôlée des monomères; et couplage des monomères résultants avec une matrice de base. La technique de polymérisation contrôlée peut être du type radicalaire contrôlée par transfert d'atome (ATRP), radicalaire contrôlée par réaction réversible d'addition-fragmentation (RAFT) ou à médiation assurée par nitroxyde (NMP). On peut ainsi élaborer des ligands bien caractérisés, utiles entre autres pour la chromatographie d'interaction hydrophobe (HIC) ou tout autre type de chromatographie.

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