Novel plant plastid promoter sequence

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/64 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2321644

A novel promoter isolated from the 5' flanking region upstream of the coding sequence of the Arabidopsis plastid clpP gene is described. Also described are a novel method for utilizing protein-coding regions of plastid genes to isolate intervening regulatory sequences and a novel method for improving plastid transformation efficiency using exogenous plastid promoters that differ in nucleotide sequence from native plastid promoters.

On décrit un nouveau promoteur isolé de la région flanquante 5' en amont de la séquence codante du gène clpP plastidial d'Arabidopsis. On décrit également un nouveau procédé d'utilisation de régions de codage de protéines de gènes plastidiaux pour isoler des séquences régulatrices interposées et un nouveau procédé permettant d'améliorer l'efficacité de la transformation plastidiale à l'aide de promoteurs plastidiaux exogènes dont la séquence nucléotidique diffère de celle des promoteurs plastidiaux natifs.

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