Synthesis of sialic acid in plants

Details Synthesis of sialic acid in plants Synthesis of sialic acid in plants

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A61K 38/14 (2006.01) C12N 5/04 (2006.01) C12N 9/10 (2006.01) C12N 9/88 (2006.01) C12N 9/90 (2006.01) C12N 15/54 (2006.01) C12N 15/60 (2006.01) C12N 15/82 (2006.01) C12P 19/02 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2649134

A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5 Ac synthase, a CMP- Neu5Ac transporter and a sialyltransferase.

L'invention concerne un procédé de synthèse d'acide sialique dans des végétaux, et des végétaux capables de synthétiser l'acide sialique. En outre, l'invention concerne également un procédé de production d'une protéine sialylée dans un végétal. Le procédé pour synthétiser l'acide sialique comprend un végétal comprenant une séquence de nucléotides codant pour une synthase d'acide N-acétyl neuraminique (Neu5Ac) ou une lyase Neu5Ac, et exprimant la séquence de nucléotides synthétisant de ce fait l'acide sialique. Le végétal peut également co-exprimer une séquence de nucléotides codant pour une ou plus d'une parmi une épimérase, une synthase CMP-Neu5 Ac, un transporteur CMP-Neu5Ac et une sialyltransférase.

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