Monochrome multiplex quantitative pcr

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2748265

Disclosed herein are methods and composi-tions for determining the copy number of a first target nu-cleic acid as compared to the copy number of a second tar-get nucleic acid in a single well with a single detection la-bel. For example, disclosed herein are methods and com-positions for determining the copy number of a first target nucleic acid as compared to the copy number of a second target nucleic acid by a monochrome multiplex quantita-tive PCR (MMQPCR) in a single well with a single detec-tion label.

L'invention concerne des procédés et des compositions permettant de déterminer le nombre de copies d'un premier acide nucléique cible comparativement au nombre de copies d'un second acide nucléique cible dans un seul puits avec une seule étiquette de détection. Par exemple l'invention concerne des procédés et des compositions permettant de déterminer le nombre de copies d'un premier acide nucléique cible comparativement au nombre de copies d'un second acide nucléique cible par une PCR quantitative multiplexe monochrome (MMQPCR) dans un seul puits avec une seule étiquette de détection.

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