Synchronized donor cells for bovine nuclear transfer

Details Synchronized donor cells for bovine nuclear transfer Synchronized donor cells for bovine nuclear transfer

C - Chemistry, Metallurgy

12

N

C12N 5/02 (2006.01) A61D 19/00 (2006.01) C12N 5/06 (2006.01) C12N 5/10 (2006.01) C12N 15/06 (2006.01)

Patent

CA 2092258

SYNCHRONIZED DONOR CELLS FOR BOVINE NUCLEAR TRANSFER Abstract A method of multiplying bovine embryos by nuclear transfer includes the steps of synchronizing donor embryonic cells at a cell cycle phase using a cell cycle inhibitor, releasing the cells from inhibition, and allowing the cells to progress into the S-phase of the cell cycle before fusion to recipient oocytes. In a preferred process, the recipient oocyte is activated before fusion such that both donor and recipient are in S-phase when fusion occurs. More preferably, the cell cycles of both donor and recipient are coordinated before fusion such that both donor and recipient would re-enter M-phase at about the same time. The method is intended to increase the percentage of viable bovine nuclear transfer embryos to a percentage higher than can be obtained without synchronization of donor nuclei.

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